Dr. Shakhawat Bhuiyan
Associate Professor of Biology
B.S., 1990, Biochemistry, University of Dhaka
M.S., 1992, Microbiology, University of Dhaka
Ph.D., 1999, Applied Microbiology, Ehime University
Office: Meyer S-13
General Microbiology (BIOL 3400), General Genetics (BIOL 3401), Cell & Molecular Biology (BIOL 4403), Botany (BIOL 4410), Nutrition (NUTR 3300), Topical Seminar (BIOL 4499), Undergraduate Research (BIOL 4490)
My research addresses on signal recognition particle (SRP), an important cytosolic ribonucleoprotein complex, which directs secretory proteins to and across biological membranes in all organisms. Proteins are essential to structure and function of the body. All secretory proteins are synthesized in the cytosol and must be transported to proper cellular compartments. Proper cell function depends on correct protein localization. In all cells, SRP targets secretory proteins for the co-translational delivery across the ER-membrane by binding to hydrophobic signal sequences at N-terminus of polypeptides as they emerge from ribosome. This step is a prerequisite for the subsequent sequestering of many proteins including those, which play a vital role in many human diseases. The recognition of the signal peptide is carried out by the large domain of the SRP protein SRP54M, and SRP RNA helix 8b. The goal of this research is to enrich scientific understanding of the molecular mechanism of signal peptide interactions within the signal recognition particle. Student research, under my supervision will focus on molecular cloning, expression and reconstitution of SRP components, especially their interaction within the signal peptide. The students participate in the project has been exposed to most up-to-date molecular cloning, protein expression & purification techniques.
I am Principal Investigator (PI) of the UNIMET cluster grant entitled “Molecular Signal Peptide Recognition and Immune Dysfunction in Space Environment” sponsored by the United Negro College Funds Special Programs Corporation (UNCFSP) and NASA to carry out research on signal recognition particle with the participation of undergraduate students. This grant also presents an excellent opportunity to collaborate with UNIMET cluster investigators from other institutes such as Dr. Marriott of Savannah State University, Dr. Sundaresan of Texas Southern University, Dr. Mao of Tougaloo College, and NASA scientist Dr. Pamela Denkins (JSC). I am also research mentor for undergraduate students who are working under Science, Mathematics and Research Training (SMaRT) grant, NSF. I supervised several undergraduate students for their independent research funded through my UNIMET and NSF grants. Undergraduate students who worked with me presented their research in different scientific conferences. I have been collaborating with the researchers at the University of Texas Health Center at Tyler, TX, and University of Toledo, OH that resulted in several peer-reviewed publications over the past five years.
Zwieb, C. and Bhuiyan, S. 2010. Archaeal signal recognition particle shows the way. Archaea 2010, Article ID 485051, 11 pages.Liu, T.-A., Bhuiyan, S., Liu, M.-Y., Sugahara, T., Sakakibara, Y., Suiko, M., Yasuda, S., Kakuta, Y., Kimura, M., Williams, F.E., and Liu, M.-C., 2010. Zebrafish as a Model for the Study of the Phase II Cytosolic Sulfotransferases. Current Drug Metabolism11, 538-546.
Yasuda, S., Burgess, M., Yasuda, T., Liu, M.-Y., Bhuiyan, S., Williams, F.E., Kurogi, K., Sakakibara, Y.,
Suiko, M. and Liu, M.-C. 2009. A Novel Hydroxysteroid-sulfating Cytosolic Sulfotransferase, SULT3 ST3, from Zebrafish: Identification, Characterization, and Ontogenic Study. Drug Metabolism Letters 3:217-227.
Ilangovan, U., Bhuiyan, S. H., Hinck, S. C., Hoyle, T. J., Pakhomova, N. O., Zwieb, C. and Hinck, P. A. 2008. A. fulgidus SRP54M-domain. Journal of Biomolecular NMR, 41:241-248.
Tzu-An Liu, Shakhawat Bhuiyan , Rhodora Snow, Shin Yasuda, Tomoko Yasuda, Yuh-Shyong Yang,Frederick E. Williams, Ming-Yih Liu, Glendora Carter, Ming-Cheh Liu. 2008. Identification and Characterization of Two Novel Cytosolic Sulfotransferases, SULT1 ST7 and SULT1 ST8, from Zebrafish. Aquatic Toxicology, 89:94-102.
Yasuda, T., Yasuda, S., Williams, F.E., Bhuiyan, S. H., Carter, G., Snow, R., Liu, M.-C. 2008.
Characterization and ontogenic study of novel steroid-sulfating SULT3 sulfotranferases from zebrafish. Molecular and Cellular Endocrinology, 294:29-36.
Iakhiaeva, E., Bhuiyan, S. H., Yin, J. and Zwieb, C. 2006. Protein SRP68 of human signal recognition particle: identification of the RNA and SRP72 binding domains. Protein Sci. 15:1290-302.